Description
This module introduces the students to essential laboratory techniques. A mix of theoretical and practical sessions will equip the students with the necessary knowledge and experience to perform a range of common research techniques including:
Cloning: RNA extraction, cDNA Reverse transcription, Polymerase Chain Reaction (PCR), Ligation, Transformation, Colony Screening, DNA Extraction, Restriction Enzyme digestion, DNA mapping.
Tissue Culture: Aseptic techniques, Cell counting, Cell Subculturing, Transfection.
Protein expression: ELISA, Western blotting and protein quantification.
The students will also learn how to use ENSMBL to interrogate and manipulate gene sequences and how to keep a lab book, in order to properly archive protocols and present their data in a useful and clear way.
The skills acquired by the students in this module should be of use to them throughout their research career.
Module deliveries for 2024/25 academic year
Last updated
This module description was last updated on 19th August 2024.
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